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ORIGINAL ARTICLE
Year : 2011  |  Volume : 6  |  Issue : 1  |  Page : 5-7

Specular Microscopic Features of Corneal Endothelial Vacuolation


Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Correspondence Address:
Mozhgan Rezaei Kanavi
Assistant Professor of Ophthalmology, Ophthalmic Research Center, #23 Paidarfard St., Boostan 9 St., Pasdaran, Tehran 16666
Iran
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Source of Support: None, Conflict of Interest: None


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Purpose: To introduce a specular microscopic reference image for endothelial vacuolation in donated corneas. Methods: Two corneas from a donor with diffuse, round to oval dark areas at the endothelial level on slit lamp biomicroscopy and one normal-appearing donor cornea underwent specular microscopy, histopathologic evaluation and transmission electron microscopy. Results: Specular microscopy of the two corneas with abnormal-looking endothelium revealed large numbers of dark, round to oval structures within the endothelium in favor of endothelial vacuolation. Light microscopy disclosed variable sized cyst-like structures within the cytoplasm. Transmission electron microscopy showed electronlucent and relatively large-sized intracytoplasmic vacuoles. These features were not observed in the endothelium of the normal cornea. Conclusion: The specular microscopic features of endothelial vacuolation in donated corneas were confirmed by light microscopy and transmission electron microscopy, therefore the specular image may be proposed as a reference to eye banks.


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