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ORIGINAL ARTICLE
Year : 2013  |  Volume : 8  |  Issue : 1  |  Page : 4-8

Knockdown of the Placental Growth Factor Gene Inhibits Laser Induced Choroidal Neovascularization in a Murine Model


1 Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
3 Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran
4 Iranian Blood Transfusion Organization, Tehran, Iran

Correspondence Address:
Zahra-Soheila Soheili
Department of Biochemistry, National Institute of Genetic Engineering and Biotechnology, Tehran
Iran
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Source of Support: None, Conflict of Interest: None


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Purpose: To evaluate the effect of placental growth factor (PlGF) gene knockdown in a murine model of laser-induced choroidal neovascularization. Methods: Choroidal neovascularization was induced in the left eyes of 11 mice by infrared laser. Small interfering RNA (siRNA, 20 picomoles/10 μl) corresponding to PlGF mRNA was administered intravitreally by Hamilton syringe in all subjects. One month later, fluorescein angiography and histolologic examination were performed. Results: No leakage was apparent in the 11 eyes treated with siRNA cognate to PlGF. The results of histological evaluation were consistent with angiographic findings showing absence of choroidal neovascularization. Conclusion: Knockdown of the PlGF gene can inhibit the growth of laser-induced choroidal neovascularization in mice.


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