• Users Online: 1195
  • Home
  • Print this page
  • Email this page
Home Current issue Ahead of print Search About us Abstracting and Indexing Editorial board Archives Submit article Instructions Subscribe Contacts Login 


 
 Table of Contents  
REVIEW ARTICLE
Year : 2018  |  Volume : 13  |  Issue : 3  |  Page : 315-332

Optical coherence tomography angiography in eyes with retinal vein occlusion


1 Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio, USA; School of Medicine, Royal College of Surgeons in Ireland, Ireland
2 Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio, USA; Department of Ophthalmology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3 Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio, USA
4 Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio; Cleveland Clinic, Lerner College of Medicine, Case Western Reserve University, Cleveland, USA

Date of Submission10-Dec-2017
Date of Acceptance04-Jan-2018
Date of Web Publication12-Jul-2018

Correspondence Address:
Rishi P Singh
Cole eye institute, 9500 Euclid Avenue, Desk i32, Cleveland, Ohio 44195
USA
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jovr.jovr_264_17

Clinical trial registration NCT03431454

Rights and Permissions
  Abstract 


Optical coherence angiography (OCTA) is a noninvasive technique that has been introduced in recent years to detect ophthalmological pathology. The growing usage of OCTA to detect retinal abnormalities can be attributed to its advantages over the reference-standard fluorescein angiography (FA), although both of these techniques can be used in association. OCTA's advantages include its dye independency, its ability to produce depth-resolved images of retinal and choroidal vessels that yield images of different vascular layers of the retina, and the better delineation of the foveal avascular zone. OCTA's disadvantages include the lack of normalized patient data, artefactual projection issues, and its inability to detect low-flow lesions or pathologic conditions. Different OCTA platforms use unique algorithms to detect microvasculature, which are implemented in both spectral-domain (SD) and swept-source (SS) OCT machines. Microvascular changes in retinal vein occlusions (RVOs) are visible in both the superficial and deep capillary networks of the retina in OCTA. These visualizations include a decrease in foveal and parafoveal vascular densities, non-perfusion areas, capillary engorgement and telangiectasias, vascular tortuosity, microaneurysms, disruption of the foveal perivascular plexus, and formation of collateral vessels. The restricted field of view and inability to show leakage are important limitations associated with the use of OCTA in RVO cases. In this article, we present a brief overview of OCTA and a review of the changes detectable in different slabs by OCTA in RVO cases published in PubMed and Embase.

Keywords: Macular Edema; Macular Ischemia; Optical Coherence Tomography Angiography; Retina; Retinal Vascular Disease; Retinal Vein Occlusion


How to cite this article:
Tsai G, Banaee T, Conti FF, Singh RP. Optical coherence tomography angiography in eyes with retinal vein occlusion. J Ophthalmic Vis Res 2018;13:315-32

How to cite this URL:
Tsai G, Banaee T, Conti FF, Singh RP. Optical coherence tomography angiography in eyes with retinal vein occlusion. J Ophthalmic Vis Res [serial online] 2018 [cited 2020 Apr 1];13:315-32. Available from: http://www.jovr.org/text.asp?2018/13/3/315/236506




  Introduction Top


Optical coherence tomography angiography (OCTA) has become a valuable imaging tool for the evaluation of retinal pathologies such as diabetic retinopathy, age-related macular degeneration, and retinal artery and retinal vein occlusions (RVOs). Its ability to delineate the fine microvascular detail of the retinal vasculature in the superficial and deep retinal plexus without dyes is advantageous for diagnosing retinal diseases, which will most likely lead to its widespread use in the future.[1],[2],[3]

Angiography has been part of the diagnostic work-up of RVOs. A major aim of these evaluations is to delineate the area of the ischemic retina. Fluorescein angiography (FA), the standard method, can show ischemic areas in both the central and peripheral retina, and these images have been used for both prognostication and treatment decisions in cases of RVOs. However, FA may not always yield clear images of the foveal avascular zone (FAZ), whose intactness has prognostic value.[4]

OCTA images are generated by algorithms that detect either phase and/or amplitude differences of moving parts (notably red blood cells) of successive OCT B-scans to construct an image of the vasculature.[5] As OCTA is fundamentally different from FA, the changes in RVOs detectable with this technique do not always mirror those shown by FA. Some changes are better visualized by OCTA, such as FAZ irregularities that are not obscured by leakage.[6],[7] Some changes in the microvasculature that can be evaluated by OCTA, while not visible by FA, include those in the deep capillary plexus.[8],[9] However, with the current technology, there are limitations to OCTA as well, which include a restricted field of view and inability to detect leakage. In addition, all software platforms lack normalized data to determine if the vascular patterns seen are truly abnormal or are on the normal spectrum and instead compromise of patient to patient variation. At present, several devices are equipped with OCTA functions: RTVue XR Avanti (Optovue, Inc., Fremont, CA. USA), Triton and Atlantis (Topcon, Tokyo. Japan), Cirrus HD and PLEX Elite 9000 (Carl Zeiss Meditec, Inc., Dublin, CA, USA), Spectralis (Heidelberg Engineering, Heidelberg, Germany), and RS-3000 (Nidek Co, Gamagori, Japan). Literature regarding the use of OCTA in cases of RVOs is vast, and studies have shown that OCTA findings in cases of RVOs correlate well with the clinical, anatomic, and fluorescein angiographic (FA) findings of capillary dropout, retinal atrophy, increased vessel caliber, shunt vessels (collaterals), and the presence of intraretinal edema.[10],[11],[12],[13] In this paper, we present a brief overview of OCTA and the findings reported in the English literature.

Method of Literature Search

All papers published from January 2015 to May 2017 describing the use of OCTA in RVO were identified and reviewed through a PubMed and Embase search. The keywords “OCTA,” “optical coherence angiography” or “angiography,” and “retinal vein” or “retinal vein occlusion” were applied. Relevant papers from the reference lists of articles were also included [Table 1]. Where duplicate information was present, papers with more recent publication dates or larger study groups were referenced.
Table 1: A summary of important findings in studies reporting OCTA in RVO.

Click here to view



  A Brief Discussion About Octa Top


Technology

The OCTA algorithm generates three-dimensional, en face, depth-encoded images of microvascular blood flow in the retinal and choroidal vasculatures. By using motion contrast, OCTA can portray differing reflectance patterns over time due to the phase-shift of RBCs in retinal vessels without the need for intravenous dye injection. The device shows the differences between multiple, sequential B-scans obtained at the same retinal cross-section. Notably, OCTA systems have a characteristic threshold for slowest and fastest detectable flow.[5]

OCTA techniques can be classified into three categories: 1) phase-signal-based OCTA techniques [optical coherence angiography, phase-variance OCTA], 2) amplitude/intensity-signal-based OCTA techniques [speckle-variance OCTA, correlation-mapping OCTA, split-spectrum amplitude-decorrelation angiography (SSADA)], and 3) complex-signal-based OCTA techniques [optical microangiography (OMAG), Eigen-decomposition-based optical microangiography, imaginary part-based correlation-mapping OCTA, split-spectrum phase-gradient OCTA].[14] For example, the AngioVue software of the RTVue XR Avanti SD-OCT employs an SSADA algorithm, whereas the Zeiss AngioPlex uses a proprietary Optical Micro Angiography (OMAGc) algorithm that combines elements of the SSADA and phase-variance methodologies to produce images.[15],[16] The application of the SSADA algorithm improves the signal-to-noise ratio of flow detection while minimizing the scan acquisition time to optimize visualization of the retinal vasculature (Optovue, AngioRetina mode, software AngioAnalytics).[17]

Available Platforms

OCTA platforms can be broadly categorized into SD-OCT and SS-OCT instruments. The SD-OCT instruments such as Optovue's AngioVue operate at a ~840-nm wavelength, while SS-OCT devices such as Topcon's Triton use a longer ~ 1050-nm wavelength.[18]

Swept-source systems have a faster acquisition rate and are more expensive than spectral-domain systems. SD-OCT tends to run on a shorter wavelength with better retinal lateral and axial resolution. The longer wavelength light source used in SS-OCT devices may be less affected by ocular opacity and may provide a deeper imaging range through the retinal pigment epithelium (RPE) and choroid, which is a significant advantage for managing diseases below the RPE (age-related macular degeneration or polypoidal choroidal vasculopathy).[19],[20],[21],[22] In spectral-domain devices, software enhancement techniques such as improved depth imaging are used to better visualize the choroid and structures below the RPE. Both modalities have adequate imaging range for the retina.

OCTA in use

Currently, the standard field of view for the best image quality measures 3 × 3 mm, and enlarging the field of scanning in OCTA diminishes the details of flow. However, RVO can occur in an area of the retina that extends beyond the limited view scanned in OCTA. Wide-field and montage OCT provide detailed information of large or oblique lesions away from the arcade.[23] Wide-field OCTA images can be acquired by several methods: selecting an automated wide-angle single scan within the OCTA system itself (up to 12 mm) or by using an automatic stitching software such as AngioVueHD Montage within the Avanti RTVue XR (Optovue, Inc., CA, USA) or other semi-automated or manual methods of creating montages. Use of the montage technique allows operators to maintain the microvascular detail that appears at a higher resolution than that in the currently available FA images.[11],[23],[24]

A novel method using trial frames fitted with a +20 D lens has been described as an extended field imaging (EFI) technique to evaluate RVOs.[25] In this technique, researchers captured OCTA images by using RTVue XR Avanti OCT with AngioVue at a scan size of 8 × 8 mm, with and without EFI, and compared them. EFI images delineated an area 188.5% larger than those without EFI on average. The non-perfusion area was well-defined in the superficial capillary plexus (SCP), and this technique was useful for evaluating retinal ischemia in RVO, but the resolution of the image was not sufficient to study the deep capillary plexus (DCP) or microvascular changes.[25]

Comparison of OCTA and FA

Several studies have qualitatively compared OCTA with FA.[11],[26] The features of disrupted flow in vascular occlusions can be well imaged on OCTA and correlate well with the area seen on FA.[10],[11],[12],[13]

Advantages of OCTA

In comparison to FA, OCTA is fast, noninvasive, and allows improved and accurate visualization of microvascular changes. Due to the absence of leakage and tissue staining, and the better penetration of the longer wavelengths used in OCTA through intraretinal hemorrhage, OCTA allows better visualization of the microvascular abnormalities in RVO, including neovascular fronds, FAZ, and other microvascular abnormalities.[13],[17],[27],[28],[29],[30],[31]

The limited time frame in which optimal images of the capillary net can be captured under FA and the difficulty in focusing images in the presence of macular edema can complicate the use of FA to obtain quality images of the perifoveal capillaries and visualize RVOs. With OCTA, however, images can be captured rapidly, and when images are of insufficient quality, the process can be repeated immediately until good-quality images are obtained.

Depth-resolved studies of microcirculation are another big advantage of OCTA over FA. The ability of OCTA to delineate the microvascular changes and ischemia in both the SCP and DCP is a major advantage because many of the vascular changes in RVO occur in the DCP, which cannot be visualized by FA.[8],[9] Additionally, the reconstructed C scan of OCTA has a better rate of detection of macular edema than FA or SD-OCT alone.[32]

In comparison with FA, OCTA shows a superior ability to precisely delineate the vessels surrounding the FAZ in eyes with RVO compared with the fellow eyes and with healthy eyes.[6],[7] However, OCTA imaging of the perifoveal region in the normal retinal vasculature was equivalent to that of FA.[11] Both the retinal and the choroidal microvasculature can be visualized using OCTA while FA is used for observing the retinal vessels. Ultimately, angiography images come cross-registered with structural OCT B-scans. This process allows for precise correlation of the vasculature to the structural scans.[13],[33]

Limitations of OCTA

The limitations of OCTA include the small scanning areas, segmentation errors related to variations in macular anatomy, inability to determine the presence of leakage, proclivity for image artifacts caused by patient motion and shadowing from retinal pathologies such as cystoid macular edema, and a limited ability to visualize blood movement out of the detectable flow limit.[8],[34] Microaneurysms imaged with FA may not always be apparent on OCTA because they might have flow rates below the detection threshold of OCTA. There are still some challenges for its use as OCTA requires the patient to precisely fixate on a light during image acquisition (approximately 3 seconds), which may be difficult to achieve for patients with low visual acuity.[35]

OCTA's inability to visualize leakage may be both an advantage and a disadvantage, because it means that leakage does not obscure the vascular structures observed by OCTA. The co-registered structural OCT scans can provide indirect information about leakage, such as the presence of macular edema.[13]

OCTA is subject to various artifacts such as shadowing artifacts associated with intraretinal/subretinal hemorrhage, projection artifacts of the superficial retinal vessels over the deeper retinal layers, and motion artifacts. However, these errors are becoming less frequent because of the artifact correction strategies that are being currently implemented.[36],[37],[38]

OCTA is dependent on accurate retinal segmentation to delineate retinal vessels at different levels. However, the circumstances in which the retina is sufficiently disrupted to make precise segmentation difficult may lead to inaccurate depth localization of the vessels. 13 This mistake can be easily identified by scrolling through the entire three-dimensional dataset, instead of looking at individual en face printouts. Imprecise segmentation can be adjusted by manually altering the segmentation lines.

In the presence of cystoid edema, besides problems with accurate segmentation, shadowing artifacts of the fluid in the cystoid spaces may hamper detection of capillaries and result in overestimation of the degree of non-perfusion.[33]

Changes in OCTA of Eyes with RVO

The changes visible in OCTA can be described as qualitative and quantitative

Qualitative changes

  1. Non-perfusion areas (NPAs; [Figure 1], [Figure 2], [Figure 3] to [Figure 4]):[32] These are also called grayish areas [39] and areas with decreased vascular perfusion.[6],[40] These areas are regions without visible perfused capillaries. In RVOs, they are more extensive in the DCP than in the SCP.[6],[10],[32],[33],[39],[40] These areas are more readily visible with OCTA than with FA.[12] There may be a decrease in the vascular perfusion in both the SCP and DCP of the fellow eye of RVO patients relative to normal controls, which may be a sign of previous silent RVOs in these eyes.[6] It should be noted that the absence of visible vessels in areas of non-perfusion may not be due to a total absence of flow, but may represent areas in which the blood flow has decreased below the device's detection threshold [41]
  2. Vascular tortuosity[Figure 1], [Figure 2], [Figure 3]: This is similar to what is visible in larger vessels in OCTA, and may also include kinking, angulation, and/or spiral twisting of vessels. Tortuosity is seen in both central RVOs (CRVOs) and branch RVOs (BRVOs) and in some fellow eyes [7]
  3. Collateral vessel formation[Figure 4]: This phenomenon manifests as a long vessel traversing the area with blocked perfusion, or as a bunch of tortuous vessels in the vicinity of the area with blocked perfusion. These vessels are visible in both CRVO and BRVO eyes.[6] In some cases, they could be traced to the DCP.[6] The term venous-venous anastomosis by Kashani et al appears to refer to the same finding [10]
  4. Disruption of the perifoveal capillary plexus [Figure 1], [Figure 2], [Figure 3], [Figure 4]:[39],[40] The perifoveal capillary net is distorted in ischemic maculopathies including RVOs. Disruption of the FAZ is more common in the DCP than in the SCP.[39] Coscas et al found that the degree of disruption of the perifoveal capillary network is correlated with the presence of peripheral ischemia in FA and the degree of non-perfusion in the DCP They did not find any peripheral ischemia in FA in cases with an intact perifoveal capillary network and suggested that OCTA may be a screening tool to decide whether to perform FA [32]
  5. Dilation of the capillary plexus and venous dilation[Figure 1], [Figure 2], [Figure 3]: These phenomena are more commonly seen in the DCP [7],[10],[32],[37] and better delineated by OCTA than FA.[12],[42] This manifestation is probably caused by two mechanisms: 1) an increase in the intravascular resistance, and 2) the effect of the different cytokines and growth factors produced during the disease process.[43] In the acute phase of BRVO, capillary congestion is mostly present in the DCP at the boundary of the normal retina and will partially resolve with time [7]
  6. Microaneurysms[Figure 4]: These are detected by OCTA in BRVO, and they are more common in the DCP than in the SCP.[40] They usually form at the border of NPAs, and in collateral vessels.[42] The microaneurysms within collateral vessels are a source of persistent leakage and recurrence of edema after resolution of elevated venous pressure [40]
  7. Cystoid spaces[Figure 1], [Figure 2], [Figure 3], [Figure 4]: Cystoid spaces in the SCP are more commonly seen in CRVO, and those in the DCP are more common in BRVO than in CRVO. It is easier to find macular cystoid spaces in OCTA than in OCT and FA.[32] Cystoid spaces have no signal and coincide with areas of perfusion abnormalities.[10] This is not a universal phenomenon and there are areas with impaired perfusion without development of edema.[10] There are two explanations for the absence of OCTA signals in the area of cystoid spaces.[44] The first is the displacement of capillaries by cysts, which is favored by the observation of an increase in vascular perfusion indices after treatment in some studies.[39] The second is the development of cysts in non-perfused areas.[10] The previously described “hyper-reflective” cystoid spaces appear as “diffuse and splotchy” OCTA signals.[45] Kashani et al named these pockets as “edema with hard exudates” and proposed that these areas contain intraretinal fluid with high concentrations of lipids (a stage before complete absorption of the intraretinal fluid and formation of hard exudates).[10] The  Brownian movement More Details of the lipid particulate matter is the source of the OCTA signals. Because formation of hard exudates is a harbinger of a reduction in vision, this finding may have prognostic value
  8. Intraretinal hemorrhages: The shadowing effect of intraretinal hemorrhages may obscure images of one or both intraretinal vascular plexuses, and the level of the hemorrhage can be determined from the degree to which the images are obscured: if images of both plexuses are obscured, then the hemorrhage is above both; if neither image is obscured, it is beneath both; and if only the image of the DCP is obscured, then it lies between the two vascular plexuses [10]
  9. Non-perfused ghost vessels: These can be diagnosed when a vessel is visible on the en face OCT image, but is not detectable in OCTA. These vessels also cannot be seen in FA [46]
  10. Optic disc venous collaterals (OVCs) and neovascularization of disc (NVD):[33] OCTA shows optic disc collaterals at the level of the superficial peripapillary plexus whereas neovascular vessels are visible above the retina at the level of the vitreous. OVCs are loopy vessels whereas new vessels are a mesh of fine vessels. OCTA delineates OVCs better than both fundus photographs and FA [47]
  11. Neovascularization elsewhere (NVE): This phenomenon can be detected using OCTA, and the visibility of new vessels with OCTA is greater than that with FA because of the absence of leakage in OCTA.[48] This modality may enable physicians to perform quantitative follow-up of new vessels and evaluate the response to treatment.
Figure 1: OCTA (3 × 3 mm) in a case of CRVO. (a) OCTA at the level of the superficial capillary plexus (SCP) showing vascular tortuosity, dilation and telangiectasia (arrow) along with decreased vascular density and non-perfusion areas. Also note the irregular and enlarged foveal avascular zone. (b) En face OCT at the level of the SCP shows the presence of cystoid edema, which corresponds to dark circular areas without vessel signals in OCTA (arrowhead in A). (c) B-scan OCT with perfusion overlay and segmentation lines. (d) Color-coded vascular density map. (e) Numerical report of the vascular density.

Click here to view
Figure 2: OCTA (3 × 3 mm) in a case of CRVO. (a) OCTA at the level of the deep capillary plexus (DCP) showing vascular tortuosity, dilation, and telangiectasia (arrow) along with decreased vascular density and non-perfusion areas. Also note the irregular and enlarged foveal avascular zone. (b) En face OCT at the level of the DCP. Note the presence of cystoid edema corresponding to dark circular areas without vessel signals in OCTA (arrowheads in a). (c) B-scan OCT with perfusion overlay and segmentation lines. (d) Color-coded vascular density map. (e) Numerical report of the vascular density.

Click here to view
Figure 3: OCTA (6 × 6 mm) of a case of BRVO. (a) OCTA at the level of the superficial capillary plexus (SCP) showing vascular tortuosity, dilation and telangiectasia (arrow) along with decreased vascular density and non-perfusion areas in the superotemporal region (a). (b) En face OCT at the level of the SCP. Note the presence of cystoid edema corresponding to dark circular areas without vessel signals in OCTA (arrowhead in a). (c) B-scan OCT with perfusion overlay and segmentation lines showing the level of OCTA in (a). (d) Color-coded vascular density map. (e) Numerical report of the vascular density.

Click here to view
Figure 4: OCTA (6 × 6 mm) of a case of BRVO. (a) OCTA at the level of the deep capillary plexus (DCP). Shunt vessels (arrow) and microaneurysms (arrowheads) are visible in the superotemporal area along with decreased vascular density and non-perfusion areas (a). (b) En face OCT at the level of the DCP. (c) B-scan OCT with perfusion overlay and segmentation lines showing the level of OCTA in (a). (d) Color-coded vascular density map. (e) Numerical report of the vascular density.

Click here to view


Quantitative Changes

a. Foveal and perifoveal vascular density: Vascular density both in the foveal and parafoveal areas and all over the scanned area have been reported to be lower in RVO eyes relative to those in control eyes. However, there are different results regarding the layers that are affected in each type of RVO.[7],[40],[49] Due to the wide variations in foveal vascular density (FVD) in normal individuals, this measure may not always be affected in RVOs.[39]

Vascular perfusion density is another significant factor associated with photoreceptor integrity and visual acuity.[50],[51] Changes in vascular density in the presence of macular edema have also been reported, but the results of different studies differ. Mastropasqua et al reported significant positive correlations between macular thickness and the vascular density in superficial, deep, and choriocapillaris plexuses, and this correlation has been ascribed to the high levels of VEGF, which increases both the macular thickness and the vascular diameter, thereby increasing the percentage of reported flow pixels by the instrument.[40] Meanwhile, Koulisis et al described decreased SCP vascular density in the presence of macular edema due to RVO compared to that in eyes without edema, while DCP and non-segmented vascular densities were not affected.[49] This discrepancy may be due to the different inclusion criteria for macular edema or different OCTA platforms used. Seknazi et al, in a retrospective study, found that a vascular density of less than 46% in the DCP in eyes with CRVO is the limit below which peripheral retinal non-perfusion becomes probable and suggested the use of this limit as an indication for performing FA in CRVO patients.[51]

  1. Measurement of NPA: In a study involving manual measurement of NPA in the parafoveal area, this parameter was found to be the most significant factor associated with VA and macular sensitivity in microperimetry in eyes with RVO, and was even more significant than the ellipsoid zone (EZ) continuity.[52] Qualitative grading of non-perfusion in both plexuses was also reported to be significantly correlated with peripheral non-perfusion [51]
  2. Measurement of FAZ: Despite the variability of FAZ size in normal individuals,[53],[54] the FAZ is enlarged in the DCP of RVO eyes relative to those in normal controls and fellow eyes, and relative to the FAZ of the SCP.[7],[42],[54] FAZ findings of the SCP may vary. While Rispoli et al and Casselholmde Salles et al found an enlargement of the SCP ischemic area,[7],[54] Suzuki et al reported no significant alterations.[42] Casselholmde Salles et al also reported an association between EZ disruption and the superficial FAZ area.[54]


To reduce the artifacts from segmentation errors in eyes with macular edema, Adhi et al used non-segmented OCTA images for calculation of the FAZ. FAZ enlargement was reported in comparison to both fellow eyes and normal controls.[6]

Suzuki et al reported that the FAZ was larger in eyes with CRVO than in eyes with BRVO. The authors proposed that FAZ size may be related to the intraocular VEGF levels, as they found larger FAZs in both plexuses in eyes receiving fewer intraocular injections.[42]

OCTA Parameters Found to be Associated with VA in Eyes with RVO

The FAZ area in the SCP,[54],[55] FAZ maximum diameter,[56],[57] NPA and the PFVD in the DCP,[52],[58] and the DCP vascular perfusion are the factors found to be associated with BCVA.[42] Even though Mastropasqua et al did not find any correlations between vascular perfusion in the SCP, DCP, VA, and microperimetric indices,[40] Manabe et al reported decreased retinal sensitivity over areas with vascular non-perfusion in both the SCP and DCP, with a stronger correlation with non-perfusion in the SCP.[59]

Changes in the OCTA Findings of RVO after Treatment

The qualitative changes of vascular telangiectasia and dilation, and perifoveal vascular disruption have been reported to improve after treatment with both anti-VEGFs and steroid implants.[39],[40],[60] However, the vascular density (VD) of both the SCP and DCP either remained unchanged or reduced after treatment. This may be due to either the continued expansion of vascular non-perfusion over time, as is observed during conversion from non-ischemic to ischemic CRVO, or the nonreversible ischemic damage of the retinal vessels.[39],[40],[60]

Suzuki et al reported on FAZ alterations after anti-VEGF treatment in both SCP and DCP, with a greater increase in the SCP despite the concomitant improvement in vision. It is interesting that the degree of FAZ enlargement was bigger in eyes that received fewer anti-VEGF injections.[42]

Reduction of the NPA area in both the SCP and DCP, with a greater decrease in the DCP, has also been reported after anti-VEGF treatment. This effect was more pronounced in eyes receiving more injections. The authors attributed this to reperfusion of temporarily closed vessels by leukostasis.[42] In the same study, the vascular perfusion area (the flow area as stated in the paper) was smaller in RVO eyes than in fellow eyes, and improved in eyes that received frequent anti-VEGF injections, with a greater improvement noted in the DCP. Vascular densities do not show any changes after a single anti-VEGF injection.[61]

Spaide et al studied OCTA images of RVO eyes after volume rendering and found that cystoid spaces are formed at locations of disturbed vascular flow in the SCP, and absent or severely disturbed vascular flow in the DCP. There were no changes in the pattern of the SCP or DCP after resolution of cystoid spaces with treatment, and in cases of recurrence, cystoid spaces reformed in the same areas as previously affected.[62]

Tsuboi et al found that the presence of isolated preserved vessels in the SCP over areas of NPA in the DCP (the prefusion gap between the two layers) showed the best correlation with persistent edema.[56]

Artifacts in the OCTA Images of RVO Eyes

There are multiple sources of artifacts in OCTA images of RVO eyes. The first is the attenuation of signals due to shadowing artifacts of edema or hemorrhage, which results in overestimation of the reduction in vascular perfusion. This may be the cause of the reported rarefaction of the choriocapillaris vascular perfusion in the affected sector of BRVO and under the fovea in CRVO, which improved after treatment with steroid.[40] Difficulty and inaccuracy in segmentation of slabs in OCTA, may lead to difficulty in finding the SCP and DCP in addition to under-sampling the deep vascular plexus.[62] Excessive movement due to poor vision causes significant motion artifacts in OCTA. In a study, 18% of 3 × 3 OCTA images in RVO cases were unreadable, and the strongest predictor for a poor-quality OCTA image was low vision.[35]


  Discussion Top


OCTA is a relatively new noninvasive modality for imaging retinal blood flow. It is based on detection of the motion of blood constituents in OCT images and provides better visualization of the macular capillaries and FAZ relative to FA.

The microvascular changes associated with RVOs in the posterior pole, including NPAs, vascular tortuosity and telangiectasia, disruption of the perifoveal capillary net, and formation of microaneurysms and collaterals, are all readily visible in OCTA images. OCTA is of great help in differentiating between optic disc collateral vessels and neovascular fronds. This technique is better than both FA and OCT in visualizing the microvascular changes and even the cystoid spaces. Currently the most notable limitation of OCTA in RVO cases seems to be the inability to view peripheral vascular perfusion, which is a significant factor in management of these eyes. Montage images, wide-field OCT imaging, and extended field techniques are some solutions that have thus far been proposed, but none of them is still optimal enough for routine clinical use.

The microvascular changes in RVO are more prominent in the DCP than the SCP, which can be described by the architectural organization of vessels in these two plexuses.[6],[39],[58] DCP is composed of capillaries with a vortex configuration, the center of which is aligned with the course of venules in the SCP.[63] Thus, it seems that the DCP drains into the larger superficial veins. This vessel configuration has previously been reported in animals.[64] Thus, the increase in intravenous pressure in RVO is directly transmitted to the DCP.[64] Besides the direct connection of the superficial capillaries to the retinal arterioles, this provides the capillaries with higher perfusion pressure and oxygenation, which may somewhat protect them from the ischemic changes of increased venous pressure. Quantitative measurements of the FAZ and NPA areas, vascular density, perfusion in SCP and DCP, their correlation with function of the macula, and their changes after treatment are areas of active research in RVO. OCTA will not only help us understand changes in the complex microvasculature of macula after vein occlusion, but will certainly have an undeniable role in the management of RVO patients in the future.

Financial Support and Sponsorship

Dr. Singh has received grants from Alcon and Apellis; grants and personal fees from Regeneron, Genentech, and Zeiss; and personal fees from Shire and Optos during the conduct of the study.

Conflict of Interest

There are no conflicts of interest.



 
  References Top

1.
Bonini Filho MA, Adhi M, de Carlo TE, Ferrara D, Baumal CR, Witkin AJ, et al. Optical coherence tomography angiography in retinal artery occlusion. Retina 2015;35:2339-2346.  Back to cited text no. 1
    
2.
Freiberg FJ, Pfau M, Wons J, Wirth MA, Becker MD, Michels S. Optical coherence tomography angiography of the foveal avascular zone in diabetic retinopathy. Graefes Arch Clin Exp Ophthalmol 2016;254:1051-1058.  Back to cited text no. 2
    
3.
Sarraf D, Rahimy E, Fawzi AA, Sohn E, Barbazetto I, Zacks DN, et al. Paracentral acute middle maculopathy: A new variant of acute macular neuroretinopathy associated with retinal capillary ischemia. JAMA Ophthalmol 2013;131:1275-1287.  Back to cited text no. 3
    
4.
Balaratnasingam C, Inoue M, Ahn S, McCann J, Dhrami-Gavazi E, Yannuzzi LA, et al. Visual acuity is correlated with the area of the foveal avascular zone in diabetic retinopathy and retinal vein occlusion. Ophthalmology 2016;123:2352-2367.  Back to cited text no. 4
    
5.
Moult E, Choi W, Waheed NK, Adhi M, Lee B, Lu CD, et al. Ultrahigh-speed swept-source OCT angiography in exudative AMD. Ophthalmic Surg Lasers Imaging Retina 2014;45:496-505.  Back to cited text no. 5
    
6.
Adhi M, Filho MAB, Louzada RN, Kuehlewein L, de Carlo TE, Baumal CR, et al. Retinal capillary network and foveal avascular zone in eyes with vein occlusion and fellow eyes analyzed with optical coherence tomography angiographyretinal capillary network and FAZ in RVO with OCTA. Investig Ophthalmol Vis Sci 2016;57:OCT486-94.  Back to cited text no. 6
    
7.
Rispoli M, Savastano MC, Lumbroso B. Capillary network anomalies in branch retinal vein occlusion on optical coherence tomography angiography. Retina 2015;35:2332-2338.  Back to cited text no. 7
    
8.
de Carlo TE, Romano A, Waheed NK, Duker JS. A review of optical coherence tomography angiography (OCTA). Int J Retina Vitreous 2015;1:5.  Back to cited text no. 8
    
9.
Savastano MC, Lumbroso B, Rispoli M. In vivo characterization of retinal vascularization morphology using optical coherence tomography angiography. Retina 2015;35:2196-2203.  Back to cited text no. 9
    
10.
Kashani AH, Lee SY, Moshfeghi A, Durbin MK, Puliafito CA. Optical coherence tomography angiography of retinal venous occlusion. Retina 2015;35:2323-2331.  Back to cited text no. 10
    
11.
Matsunaga D, Yi J, Puliafito CA, Kashani AH. OCT angiography in healthy human subjects. Ophthalmic Surg Lasers Imaging Retina 2014;45:510-515.  Back to cited text no. 11
    
12.
Suzuki N, Hirano Y, Yoshida M, Tomiyasu T, Uemura A, Yasukawa T, et al. Microvascular abnormalities on optical coherence tomography angiography in macular edema associated with branch retinal vein occlusion. Am J Ophthalmol 2016;161:126-32 e1.  Back to cited text no. 12
    
13.
Waheed NK, De Carlo TE, Chin AT, Duker JS. OCT angiography in retinal diagnosis and treatment. Retinal Phys 2015;12:26-42.  Back to cited text no. 13
    
14.
Chen CL, Wang RK. Optical coherence tomography based angiography [Invited]. Biomed Opt Express 2017;8:1056-1082.  Back to cited text no. 14
    
15.
Zeiss. AngioPlex OCT Angiography. v. 2017. Available from: https://www.zeiss.com/meditec/us/c/oct-angiography.html. [Last Accessed on 2017 Sep 01].  Back to cited text no. 15
    
16.
Optovue. AngioVue. v. 2017. Available from: https://www.optovue.com/products/angiovue/. [Last Accessed on 2017 Sep 01].  Back to cited text no. 16
    
17.
Baumal CR. In: Bandello F, Souied E, Querques G, editors. OCT Angiography in Retinal and Macular Dseases. Basel, Switzerland: Karger; 2016; v. 56.  Back to cited text no. 17
    
18.
Potsaid B, Baumann B, Huang D, Barry S, Cable AE, Schuman JS, et al. Ultrahigh speed 1050nm swept source/Fourier domain OCT retinal and anterior segment imaging at 100,000 to 400,000 axial scans per se cond. Opt Express 2010;18:20029-20048.  Back to cited text no. 18
    
19.
Povazay B, Hermann B, Unterhuber A, Hofer B, Sattmann H, Zeiler F, et al. Three-dimensional optical coherence tomography at 1050 nm versus 800 nm in retinal pathologies: Enhanced performance and choroidal penetration in cataract patients. J Biomed Opt 2007;12:041211.  Back to cited text no. 19
    
20.
Saito M, Iida T, Nagayama D. Cross-sectional and en face optical coherence tomographic features of polypoidal choroidal vasculopathy. Retina 2008;28:459-464.  Back to cited text no. 20
    
21.
Ueno C, Gomi F, Sawa M, Nishida K. Correlation of indocyanine green angiography and optical coherence tomography findings after intravitreal ranibizumab for polypoidal choroidal vasculopathy. Retina 2012;32:2006-2013.  Back to cited text no. 21
    
22.
Unterhuber A, Považay B, Hermann B, Sattmann H, Chavez-Pirson A, Drexler W. In vivo retinal optical coherence tomography at 1040 nm-enhanced penetration into the choroid. Opt Express 2005;13:3252-3258.  Back to cited text no. 22
    
23.
de Carlo TE, Salz DA, Waheed NK, Baumal CR, Duker JS, Witkin AJ. Visualization of the retinal vasculature using wide-field montage optical coherence tomography angiography. Ophthalmic Surg Lasers Imaging Retina 2015;46:611-616.  Back to cited text no. 23
    
24.
Choi W, Mohler KJ, Potsaid B, Lu CD, Liu JJ, Jayaraman V, et al. Choriocapillaris and choroidal microvasculature imaging with ultrahigh speed OCT angiography. PLoS One 2013;8:e81499.  Back to cited text no. 24
    
25.
Kimura M, Nozaki M, Yoshida M, Ogura Y. Wide-field optical coherence tomography angiography using extended field imaging technique to evaluate the nonperfusion area in retinal vein occlusion. Clin Ophthalmol 2016;10:1291-1295.  Back to cited text no. 25
    
26.
Spaide RF, Klancnik JM, Jr., Cooney MJ. Retinal vascular layers imaged by fluorescein angiography and optical coherence tomography angiography. JAMA Ophthalmol 2015;133:45-50.  Back to cited text no. 26
    
27.
de Carlo TE, Bonini Filho MA, Baumal CR, Reichel E, Rogers A, Witkin AJ, et al. Evaluation of preretinal neovascularization in proliferative diabetic retinopathy using optical coherence tomography angiography. Ophthalmic Surg Lasers Imaging Retina 2016;47:115-119.  Back to cited text no. 27
    
28.
Rispoli M, Antonio L, Mastropasqua L, Lumbroso B. Angiography Version 2.0. Retina Today 2016:74-82.  Back to cited text no. 28
    
29.
Novais EA, Adhi M, Moult EM, Louzada RN, Cole ED, Husvogt L, et al. Choroidal neovascularization analyzed on ultrahigh-speed swept-source optical coherence tomography angiography compared to spectral-domain optical coherence tomography angiography. Am J Ophthalmol 2016;164:80-88.  Back to cited text no. 29
    
30.
Salz DA, de Carlo TE, Adhi M, Moult E, Choi WJ, Baumal CR, et al. Select features of diabetic retinopathy on swept-source optical coherence tomographic angiography compared with fluorescein angiography and normal eyes. JAMA Ophthalmol 2016;134:644-650.  Back to cited text no. 30
    
31.
Stanga PE, Papayannis A, Tsamis E, Stringa F, Cole T, D'Souza Y, et al. New findings in diabetic maculopathy and proliferative disease by swept-source optical coherence tomography angiography. Dev Ophthalmol 2016;56:113-121.  Back to cited text no. 31
    
32.
Coscas F, Glacet-Bernard A, Miere A, Caillaux V, Uzzan J, Lupidi M, et al. Optical coherence tomography angiography in retinal vein occlusion: Evaluation of superficial and deep capillary plexa. Am J Ophthalmol 2016;161:160-71 e1-2.  Back to cited text no. 32
    
33.
Novais EA, Waheed NK. Optical coherence tomography angiography of retinal vein occlusion. Dev Ophthalmol 2016;56:132-138.  Back to cited text no. 33
    
34.
Cole ED, Novais EA, Louzada RN, Moult EM, Lee BK, Witkin AJ, et al. Visualization of changes in the choriocapillaris, choroidal vessels, and retinal morphology after focal laser photocoagulation using OCT angiography. Invest Ophthalmol Vis Sci 2016;57:OCT356-61.  Back to cited text no. 34
    
35.
Nobre-Cardoso J, Keane PA, Sim DA, Bradley P, Agrawal R, Addison PK, et al. Systematic evaluation of optical coherence tomography angiography in retinal vein occlusion. Am J Ophthalmol 2016;163:93-107 e6.  Back to cited text no. 35
    
36.
Montuoro A, Wu J, Waldstein S, Gerendas B, Langs G, Simader C, et al. Motion artefact correction in retinal optical coherence tomography using local symmetry. Med Image Comput Comput Assit Interv 2014;8674:130-137.  Back to cited text no. 36
    
37.
Ricco S, Chen M, Ishikawa H, Wollstein G, Schuman J. Correcting motion artifacts in retinal spectral domain optical coherence tomography via image registration. Miccai. 2009;12(Pt 1):100-107.  Back to cited text no. 37
    
38.
Zawadzki RJ, Capps AG, Werner JS. Progress on developing adaptive optics-optical coherence tomography for in vivo retinal imaging: Monitoring and correction of eye motion artifacts. IEEE J Sel Top Quantum Electron 2014;20:322-333.  Back to cited text no. 38
    
39.
Glacet-Bernard A, Sellam A, Coscas F, Coscas G, Souied EH. Optical coherence tomography angiography in retinal vein occlusion treated with dexamethasone implant: A new test for follow-up evaluation. Eur J Ophthalmol 2016;26:460-468.  Back to cited text no. 39
    
40.
Mastropasqua R, Toto L, Di Antonio L, Borrelli E, Senatore A, Di Nicola M, et al. Optical coherence tomography angiography microvascular findings in macular edema due to central and branch retinal vein occlusions. Sci Rep 2017;7:40763.  Back to cited text no. 40
    
41.
Tokayer J, Jia Y, Dhalla AH, Huang D. Blood flow velocity quantification using split-spectrum amplitude-decorrelation angiography with optical coherence tomography. Biomed Opt Express 2013;4:1909-1924.  Back to cited text no. 41
    
42.
Suzuki N, Hirano Y, Tomiyasu T, Esaki Y, Uemura A, Yasukawa T, et al. Retinal hemodynamics seen on optical coherence tomography angiography before and after treatment of retinal vein occlusion. Invest Ophthalmol Vis Sci 2016;57:5681-5687.  Back to cited text no. 42
    
43.
Spaide RF. Retinal vascular cystoid macular edema: Review and new theory. Retina 2016;36:1823-1842.  Back to cited text no. 43
    
44.
Couturier A, Mane V, Bonnin S, Erginay A, Massin P, Gaudric A, et al. Capillary plexus anomalies in diabetic retinopathy on optical coherence tomography angiography. Retina 2015;35:2384-2391.  Back to cited text no. 44
    
45.
Liang MC, Vora RA, Duker JS, Reichel E. Solid-appearing retinal cysts in diabetic macular edema: A novel optical coherence tomography finding. Retin Cases Brief Rep 2013;7:255-258.  Back to cited text no. 45
    
46.
Powner MB, Sim DA, Zhu M, Nobre-Cardoso J, Jones R, Syed A, et al. Evaluation of nonperfused retinal vessels in ischemic retinopathy. Invest Ophthalmol Vis Sci 2016;57:5031-5037.  Back to cited text no. 46
    
47.
Singh A, Agarwal A, Mahajan S, Karkhur S, Singh R, Bansal R, et al. Morphological differences between optic disc collaterals and neovascularization on optical coherence tomography angiography. Graefes Arch Clin Exp Ophthalmol 2017;255:753-759.  Back to cited text no. 47
    
48.
Sogawa K, Nagaoka T, Ishibazawa A, Takahashi A, Tani T, Yoshida A. En-face optical coherence tomography angiography of neovascularization elsewhere in hemicentral retinal vein occlusion. Int Med Case Rep J 2015;8:263-266.  Back to cited text no. 48
    
49.
Koulisis N, Kim AY, Chu Z, Shahidzadeh A, Burkemper B, Olmos de Koo LC, et al. Quantitative microvascular analysis of retinal venous occlusions by spectral domain optical coherence tomography angiography. PLoS One 2017;12:e0176404.  Back to cited text no. 49
    
50.
Wakabayashi T, Sato T, Hara-Ueno C, Fukushima Y, Sayanagi K, Shiraki N, et al. Retinal microvasculature and visual acuity in eyes with branch retinal vein occlusion: Imaging analysis by optical coherence tomography angiography. Invest Ophthalmol Vis Sci 2017;58:2087-2094.  Back to cited text no. 50
    
51.
Seknazi D, Coscas F, Sellam A, Rouimi F, Coscas G, Souied EH, et al. Optical coherence tomography angiography in retinal vein occlusion: Correlations between macular vascular density, visual acuity, and peripheral nonperfusion area on fluorescein angiography. Retina 2017. doi: 10.1097/IAE.0000000000001737. [Epub ahead of print]  Back to cited text no. 51
    
52.
Kadomoto S, Muraoka Y, Ooto S, Miwa Y, Iida Y, Suzuma K, et al. Evaluation of macular ischemia in eyes with branch retinal vein occlusion: An optical coherence tomography angiography study. Retina 2017. doi: 10.1097/IAE.0000000000001541. [Epub ahead of print]  Back to cited text no. 52
    
53.
Samara WA, Say EA, Khoo CT, Higgins TP, Magrath G, Ferenczy S, et al. Correlation of foveal avascular zone size with foveal morphology in normal eyes using optical coherence tomography angiography. Retina 2015;35:2188-2195.  Back to cited text no. 53
    
54.
Casselholmde Salles M, Kvanta A, Amrén U, Epstein D. Optical coherence tomography angiography in central retinal vein occlusion: Correlation between the foveal avascular zone and visual acuity OCT angiography in CRVO. Investig Ophthalmol Vis Sci 2016;57:OCT242-6.  Back to cited text no. 54
    
55.
Chung CY, Tang HHY, Li SH, Li KKW. Differential microvascular assessment of retinal vein occlusion with coherence tomography angiography and fluorescein angiography: A blinded comparative study. Int Ophthalmol 2017. doi: 10.1007/s10792-017-0570-y. [Epub ahead of print]  Back to cited text no. 55
    
56.
Tsuboi K, Ishida Y, Kamei M. Gap in Capillary perfusion on optical coherence tomography angiography associated with persistent macular edema in branch retinal vein occlusion. Invest Ophthalmol Vis Sci 2017;58:2038-2043.  Back to cited text no. 56
    
57.
Wons J, Pfau M, Wirth MA, Freiberg FJ, Becker MD, Michels S. Optical coherence tomography angiography of the foveal avascular zone in retinal vein occlusion. Ophthalmologica 2017;235:195-202.  Back to cited text no. 57
    
58.
Kang JW, Yoo R, Jo YH, Kim HC. Correlation of microvascular structures on optical coherence tomography angiography with visual acuity in retinal vein occlusion. Retina 2017;37:1700-1709.  Back to cited text no. 58
    
59.
Manabe S, Osaka R, Nakano Y, Takasago Y, Fujita T, Shiragami C, et al. Association between parafoveal capillary nonperfusion and macular function in eyes with branch retinal vein occlusion. Retina 2017;37:1731-1737.  Back to cited text no. 59
    
60.
Sellam A, Glacet-Bernard A, Coscas F, Miere A, Coscas G, Souied EH. Qualitative and quantitative follow-up using optical coherence tomography angiography of retinal vein occlusion treated with anti-VEGF: Optical coherence tomography angiography follow-up of retinal vein occlusion. Retina 2017;37:1176-1184.  Back to cited text no. 60
    
61.
Ghasemi Falavarjani K, Iafe NA, Hubschman JP, Tsui I, Sadda SR, Sarraf D. Optical coherence tomography angiography analysis of the foveal avascular zone and macular vessel density after anti-VEGF therapy in eyes with diabetic macular edema and retinal vein occlusion. Invest Ophthalmol Vis Sci 2017;58:30-34.  Back to cited text no. 61
    
62.
Spaide RF. Volume-rendered angiographic and structural optical coherence tomography. Retina 2015;35:2181-2187.  Back to cited text no. 62
    
63.
Bonnin S, Mane V, Couturier A, Julien M, Paques M, Tadayoni R, et al. New insight into the macular deep vascular plexus imaged by optical coherence tomography angiography. Retina 2015;35:2347-2352.  Back to cited text no. 63
    
64.
Paques M, Tadayoni R, Sercombe R, Laurent P, Genevois O, Gaudric A, et al. Structural and hemodynamic analysis of the mouse retinal microcirculation. Invest Ophthalmol Vis Sci 2003;44:4960-4967.  Back to cited text no. 64
    


    Figures

  [Figure 1], [Figure 2], [Figure 3], [Figure 4]
 
 
    Tables

  [Table 1]



 

Top
 
 
  Search
 
Similar in PUBMED
 Related articles
Access Statistics
Email Alert *
Add to My List *
* Registration required (free)

 
  In this article
Abstract
Introduction
A Brief Discussi...
Discussion
References
Article Figures
Article Tables

 Article Access Statistics
    Viewed1614    
    Printed26    
    Emailed0    
    PDF Downloaded277    
    Comments [Add]    

Recommend this journal


[TAG2]
[TAG3]
[TAG4]