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  Indian J Med Microbiol
 

Figure 3. Immunocytochemistry results for Chx10, Ki67+, Oct4 and Pax6+ in established hRPE cell line. (a) Nuclei of the cells were stained blue using DAPI. (b) 90% of cells in established hRPE cell line expressed Chx10. (c) Merged a and b. (d) DAPI stained blue cell nuclei. (e) 60% of cells in established hRPE cell line expressed Ki67+. (f) Merged d and e. (g) Cell nuclei were stained blue using DAPI, (h) 50% of cells revealed Oct4 nuclei expression in established hRPE cell line. (i) Merged g and h. (j) Nuclei of cells were stained blue using DAPI. (k) 4% of established hRPE cells were positive for Pax6+ expression in culture. (l) Merged j and k (magnification, ×400). hRPE, human retinal pigment epithelial; DAPI, diamidino-2-phenylindole.

Figure 3. Immunocytochemistry results for Chx10, Ki67+, Oct4 and Pax6+ in established hRPE cell line. (a) Nuclei of the cells were stained blue using DAPI. (b) 90% of cells in established hRPE cell line expressed Chx10. (c) Merged a and b. (d) DAPI stained blue cell nuclei. (e) 60% of cells in established hRPE cell line expressed Ki67+. (f) Merged d and e. (g) Cell nuclei were stained blue using DAPI, (h) 50% of cells revealed Oct4 nuclei expression in established hRPE cell line. (i) Merged g and h. (j) Nuclei of cells were stained blue using DAPI. (k) 4% of established hRPE cells were positive for Pax6+ expression in culture. (l) Merged j and k  (magnification, ×400). hRPE, human retinal pigment epithelial; DAPI, diamidino-2-phenylindole.