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  Indian J Med Microbiol
 

Figure 4. Negative controls in which the primary antibodies for Ki67 and Pax6 (b), Chx10 (d) Oct4 (f) were omitted, showed no immune-reactivity. (a, c and e) Nuclei of the cells were stained blue using DAPI; pictures are related to fields b, d and f respectively. (h) Shows a control without both primary and secondary antibodies. Nuclei of the cells were stained blue using DAPI (g) (magnification: ×400). DAPI, diamidino-2-phenylindole.

Figure 4. Negative controls in which the primary antibodies for Ki67 and Pax6  (b), Chx10  (d) Oct4  (f) were omitted, showed no immune-reactivity. (a, c and e) Nuclei of the cells were stained blue using DAPI; pictures are related to fields b, d and f respectively. (h) Shows a control without both primary and secondary antibodies. Nuclei of the cells were stained blue using DAPI  (g) (magnification: ×400). DAPI, diamidino-2-phenylindole.