Figure 5. Morphology of hRPE cell line on 2% alginate film gelatinized with 1% NCCM. (a) Single cells on the first day of cultures. (b-d) represent hRPE cell line 3, 5 and 6. days after the beginning of the culture respectively. (e) Giant macroscopic colonies of hRPE cell line were disengaged from alginate film 10. days later. (f) All colonies were dissociated from the alginate film 13 days later. Magnifications. (a-e: ×200 and f: ×50). hRPE, human retinal pigment epithelial; DAPI, diamidino-2-phenylindole.