Figure 6. Re-plating hRPE colonies. (a) 1 day after re-plating, evidence of colony adherence to the polystyrene were manifested. (b and c) 2 to 6. days after re-plating, hRPE cells disengaged from colony structures and started to re.adhere to culture surface. (d-f) Represent a colony of hRPE cells that partially dissociated from the culture vessel surface, a suspended colony of hRPE cells and a colony of hRPE cells that completely disengaged from the culture vessel, 9 days post re-plating, respectively. Magnifications. (a: ×200 and b-f:×100). hRPE, human retinal pigment epithelial.