Figure 9. MTT assay. hRPE cell line exhibited a significant increase in total number on alginate film as compared to polystyrene. hRPE cells represented preferred growth on 1% alginate film gelatinized with ×1 NCCM (*P value = 0. 02), 1% alginate film gelatinized with ×10 NCCM (**P value = 0.02), 2% alginate film gelatinized with ×1 NCCM (***P value = 0.00008) or 2% alginate film gelatinized with ×10 NCCM (****P value = 0.02) as compared with polystyrene (control). hRPE cell line showed the best growth rate when cultured on 2% (v/w) alginate film, gelatinized with ×1 (NCCM). hRPE, human retinal pigment epithelial; NCCM, neurobasal cell culture medium; MTT: 3_(4.5_dimethylthiazol_2yl)_2.5_diphenyltetrazolium bromide.