Tumor necrosis factor gene polymorphisms in advanced non-exudative age-related macular degeneration
Mohammad Hossein Jabbarpoor Bonyadi MD 1, Morteza Bonyadi PhD 2, Hamid Ahmadieh MD 3, Nikoo Fotuhi MS 2, Nasser Shoeibi MD 4, Saeed Saadat MD 5, Zakieh Yagubi MD 6
1 Department of Ophthalmology, Gonabad University of Medical Sciences, Gonabad; Center of Excellence for Biodiversity, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
2 Center of Excellence for Biodiversity, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
3 Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4 Retina Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
5 Department of Biostatistics and Epidemiology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
6 Department of Ophthalmology, Gonabad University of Medical Sciences, Gonabad, Iran
Mohammad Hossein Jabbarpoor Bonyadi
Department of Ophthalmology, 22nd of Bahman Hospital, Naser Khosro Street, Gonabad
Source of Support: Nil., Conflict of Interest: None
Purpose: To investigate tumor necrosis factor (TNF)-α gene polymorphisms in advanced dry-type age-related macular degeneration (AMD) in a population from Northeastern Iran.
Methods: In this case-control study, 50 patients with geographic macular atrophy and 73 gender-matched controls were enrolled. Genomic deoxyribonucleic acid (DNA) was extracted from the peripheral blood. Polymerase chain reaction was performed to analyze 2 candidate single nucleotide polymorphisms in the TNF-α gene, namely −1031 thymine (T)/cytosine (C) and −308 guanine (G)/adenine (A).
Results: The distribution of the - 1031 T/C genotype was TT, 62%; TC, 36%; CC, 2% in the patients and TT, 60%; TC, 36%; CC, 4% in the controls (P = 0.94). Genotype analysis of TNF-α −308 also revealed no significant difference in distribution between patients (G, 78%; GA, 22%; AA, 0%) and controls (GG, 74%; GA, 23%; AA, 3%) (P = 0.51). None of the haplotypes nor alleles of studied TNF-α polymorphisms were significantly associated with advanced dry-type AMD.
Conclusion: The findings of this study show that polymorphisms in the TNF-α gene, do not play an important role in dry-type AMD in the studied population.